Protein-Protein Interaction Detection: Methods and Analysis
نویسندگان
چکیده
منابع مشابه
Protein-Protein Interaction Detection: Methods and Analysis
Protein-protein interaction plays key role in predicting the protein function of target protein and drug ability of molecules. The majority of genes and proteins realize resulting phenotype functions as a set of interactions. The in vitro and in vivo methods like affinity purification, Y2H (yeast 2 hybrid), TAP (tandem affinity purification), and so forth have their own limitations like cost, t...
متن کاملConstruction and Analysis of Tissue-Specific Protein-Protein Interaction Networks in Humans
We have studied the changes in protein-protein interaction network of 38 different tissues of the human body. 123 gene expression samples from these tissues were used to construct human protein-protein interaction network. This network is then pruned using the gene expression samples of each tissue to construct different protein-protein interaction networks corresponding to different studied ti...
متن کاملUse of Immunomatrix Methods to Improve Protein-Protein Interaction Detection
Immunoprecipitation (IP) and coimmunoprecipitation (co-IP) are key techniques for studying protein-protein interactions. These methods utilize immobilized protein A or protein G to isolate antibody-bound target antigens. The main disadvantage of traditional immunoprecipitation and coimmunoprecipitation is that the conditions used to elute the precipitated antigen also release the antibody, cont...
متن کاملMethods for the detection and analysis of protein-protein interactions.
A large number of methods have been developed over the years to study protein-protein interactions. Many of these techniques are now available to the nonspecialist researcher thanks to new affordable instruments and/or resource centres. A typical protein-protein interaction study usually starts with an initial screen for novel binding partners. We start this review by describing three technique...
متن کاملProtein-protein interactions: methods for detection and analysis.
The function and activity of a protein are often modulated by other proteins with which it interacts. This review is intended as a practical guide to the analysis of such protein-protein interactions. We discuss biochemical methods such as protein affinity chromatography, affinity blotting, coimmunoprecipitation, and cross-linking; molecular biological methods such as protein probing, the two-h...
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ژورنال
عنوان ژورنال: International Journal of Proteomics
سال: 2014
ISSN: 2090-2166,2090-2174
DOI: 10.1155/2014/147648